HPLC Calculator

HPLC calculator: software for chromatographic performance evaluation and HPLC method transfer

date added
07 August 2020
41023 times
If you use this program, please cite the following references:

- D. Guillarme, D. Nguyen, S. Rudaz, J.L. Veuthey, Eur. J. Pharm. Biopharm., 2007, 66, 475-482. (PDF)
- D. Guillarme, D. Nguyen, S. Rudaz, J.L. Veuthey, Eur. J. Pharm. Biopharm., 2008, 68, 430-440. (PDF)

HPLC Calculator v3.1 (English)

Compatible system: Excel 97 2016 (Office 365)

No install is necessary, only open the file « HPLC_calculator_version_ 3-1.xlsx » with Excel.

Download (1.86 Mo)

Guidelines for method transfer in HPLC

Download (0.44 Mo)

« HPLC Calculator » is a tool developed at the LCAP to improve your HPLC analytical methods in terms of time and/or resolution. It is not an optimization software and cannot find the mobile phase conditions necessary to separate compounds of interest. Only kinetic parameters are evaluated.

1. The calculator determines chromatographic performances (i.e. efficiencies and induced backpressures) of 9 selected chromatographic columns from Agilent, Waters and Thermo-Electron and gives the best working conditions in isocratic and gradient mode, according to the selected mobile and stationary phases as well as the analyte's molecular weight.

2. Furthermore, the calculator allows the user to transfer isocratic or gradient LC methods thanks to fundamental equations of chromatography. For gradient transfer, the system dwell volume should be indicated for calculation.

HPLC Calculator could be used for any transfer between HPLC systems with different column geometries, between HPLC and UHPLC (or any other sub-2m configurations) (down-scaling), but also from analytical LC to preparative LC (up-scaling).