Biomedical and metabolomics analysis

Capillary electrophoresis and coupling to sensors for biomedical analysis and characterization of counterfeit drug

• Simultaneous approach qual/quant as: development of the multiple injection
• CE and MS (/ MS): evaluation of new coupling interfaces
• CE and fluorescence: investigation of new bypass reactive
• • CE and electrochemistry: coupling to the conductivity measurements and amperometric titration

Miniaturized sample preparation techniques for the extraction of polar compounds

• liquid micro-extractions: EME, DLLME
• solid micro-extractions online SPE μSPE
• Handling of atypical biological fluids: cerebrospinal fluid, cell cultures, seminal fluid

In vitro, in vivo toxicity studies
Neurotoxicity

• Cellular models (2D and 3D)
• Polar compounds analysis

Extended steroid profile

• Serum, plasma, urine, seminal fluid analysis (>150 compounds)
• Endocrine disruption (H295R)
• Steroidogenesis evaluation
• Environmental exposure (dioxin)

Metabolomics analysis

• Targeted/untargeted acquisition in CE and LC-MS(/MS)
• Normalization strategies
• Retention time modeling
• Database

Metabolism study

• Cellular models (HLM)
• Xenobiotics
• Phase I and II biotransformations

Experimental design

• Factor screening
• Response surface for process optimization

Validation of analytical methods

• Accuracy and uncertainty profiles
• Quality by design

Development and application of chemometric tools for metabolomics
Multivariate analysis

• Dimensionality reduction
• Knowledge extraction
• Machine learning

Clustering / Regression / Discriminant analysis / Classification
Advanced methods

• Multiblock analysis
• Multiway analysis
• Megavariate analysis of variance